Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, N-terminal 10xHis-tagged, 54.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125)

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125) — Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Purity >85%.

SKU: BCRECP-002492 Category:
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Product Specifications

Product SkuBCRECP-002492
Product DescriptionRecombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-433aa. [Accession | P9WPP1].
Uniprot No.P9WPP1
Gene Namescyp125
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-433aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoN-terminal 10xHis-tagged
Molecular weight54.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26-hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one.

Biological Context

Pathway: Steroid metabolism; cholesterol degradation

Product Specifications

Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase (cyp125) is a recombinant protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), expressed in E.coli, covering amino acids 1-433aa, with N-terminal 10xHis-tagged tag, molecular weight 54.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection (PubMed:20545858, PubMed:20843794). Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25S)-26-hydroxycholest-4-en-3-one (alcohol), (25S)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25S)-3-oxocholest-4-en-26-oate (PubMed:19846551, PubMed:20545858, PubMed:20843794). Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one (PubMed:19846551, PubMed:20545858, PubMed:20843794)

Pathway

Steroid metabolism; cholesterol degradation

Gene: cyp125  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)  |  Synonyms: Cholest-4-en-3-one 26-monooxygenase; Cholest-4-en-3-one C26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming]; Cholesterol C26-monooxygenase; Cholesterol C26-monooxygenase [(25S)-3beta-hydroxycholest-5-en-26-oate forming]; Cytochrome P450 125; Steroid C27-monooxygenase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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