Recombinant Mycobacterium tuberculosis Lanosterol 14-alpha demethylase (cyp51), Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 54.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis Lanosterol 14-alpha demethylase (cyp51)

Recombinant Mycobacterium tuberculosis Lanosterol 14-alpha demethylase (cyp51) — Sterol 14alpha-demethylase whose physiological substrate is not known. Purity >90%.

SKU: BCRECP-002497 Category:
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Product Specifications

Product SkuBCRECP-002497
Product DescriptionRecombinant Mycobacterium tuberculosis Lanosterol 14-alpha demethylase (cyp51) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-451aa. [Accession | P9WPP8].
Uniprot No.P9WPP8
Gene Namescyp51
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-451aa
SpeciesMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight54.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Sterol 14alpha-demethylase whose physiological substrate is not known. Accepts electrons from the iron-sulfur ferredoxin Fdx encoded by an adjacent gene. In vitro, catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol, to produce the 8,14-dienes stereoselectively.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Mycobacterium tuberculosis Lanosterol 14-alpha demethylase (cyp51) is a recombinant protein from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh), expressed in Baculovirus, covering amino acids 1-451aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 54.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Sterol 14alpha-demethylase whose physiological substrate is not known. Accepts electrons from the iron-sulfur ferredoxin Fdx encoded by an adjacent gene. In vitro, catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol, to produce the 8,14-dienes stereoselectively

Subcellular Location

Cytoplasm

Subunit

Homodimer

Gene: cyp51  |  Organism: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)  |  Synonyms: CYPLI; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; Sterol 14-alpha demethylase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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