Recombinant Nicotiana alata Flower-specific defensin (D1), Nicotiana alata (Winged tobacco) (Persian tobacco), E.coli, N-terminal 6xHis-SUMO-tagged, 21.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Nicotiana alata Flower-specific defensin (D1)

Recombinant Nicotiana alata Flower-specific defensin (D1) — Plant defense peptide with antifungal activity against F. Purity >85%.

SKU: BCRECP-002501 Category:
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Product Specifications

Product SkuBCRECP-002501
Product DescriptionRecombinant Nicotiana alata Flower-specific defensin (D1) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 26-72aa. [Accession | Q8GTM0].
Uniprot No.Q8GTM0
Gene NamesD1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region26-72aa
SpeciesNicotiana alata (Winged tobacco) (Persian tobacco)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight21.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.

Biological Context

Subcellular Location: Secreted; Vacuole
Tissue Specificity: Most abundant in the epidermal cell layers of the petals and sepals, within the connective cells of the anthers, and the cortical cells of the style. Not detected in the tapetum, pollen mother cells, the transmitting tissue, the vascular bundles of the anther and style or in leaves. Expressed also in ovaries, but barley detectable in roots

Product Specifications

Recombinant Nicotiana alata Flower-specific defensin (D1) is a recombinant protein from Nicotiana alata (Winged tobacco) (Persian tobacco), expressed in E.coli, covering amino acids 26-72aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 21.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera

Subcellular Location

Secreted; Vacuole

Tissue Specificity

Most abundant in the epidermal cell layers of the petals and sepals, within the connective cells of the anthers, and the cortical cells of the style. Not detected in the tapetum, pollen mother cells, the transmitting tissue, the vascular bundles of the anther and style or in leaves. Expressed also in ovaries, but barley detectable in roots

Gene: D1  |  Organism: Nicotiana alata  |  Synonyms: NaD1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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