Recombinant Escherichia coli O127:H6 Intimin (eae), partial — An inverse autotransporter. Purity >90%.
| Product Sku | BCRECP-002576 |
| Product Description | Recombinant Escherichia coli O127:H6 Intimin (eae) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 658-939aa. [Accession | P19809]. |
| Uniprot No. | P19809 |
| Gene Names | eae |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 658-939aa |
| Species | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 37.0kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
An inverse autotransporter. Adhesin, which mediates attachment to the human intestine epithelial cells.
Recombinant Escherichia coli O127:H6 Intimin (eae), partial — An inverse autotransporter. Purity >90%. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
An inverse autotransporter (PubMed:25353290, PubMed:33277518). Adhesin, which mediates attachment to the human intestine epithelial cells (PubMed:2172966). Necessary for the production of attaching and effacing lesions on infected human tissue culture cells (PubMed:2172966, PubMed:10835344). Anchored to the outer membrane by binding to peptidoglycan (PGN) via its periplasmic domain, thus helping in receptor interactions during host invasion (Probable). PGN-binding may also aid in resisting mechanical and chemical stress during transit of the bacterium through the gastrointestinal tract of the host (Probable). Periplasmic domain binds purified E.coli PGN sacculi under acidic conditions in vitro and in vivo, but does not bind to chitin (PubMed:25353290). Periplasmic domain binds PGN sacculi with an apparent dissociation constant (Kd) of 0.8 uM (PubMed:25353290). No binding to PGN in vitro at normal physiological pH 7.4 (PubMed:25353290)
Cell outer membrane
Homodimer (PubMed:25353290). Interacts with Tir (PubMed:10835344, PubMed:10890451)
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
