Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1), partial, Biotinylated, Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 67.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1), partial, Biotinylated

Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1), partial, Biotinylated — Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. Purity >85%.

SKU: BCRECP-002579 Categories: , ,
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Product Specifications

Product SkuBCRECP-002579
Product DescriptionRecombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 458-641aa. [Accession | P03211].
Uniprot No.P03211
Gene NamesEBNA1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region458-641aa
SpeciesEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight67.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency.

Product Specifications

Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 1 (EBNA1), partial, Biotinylated — Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection (PubMed:15479791, PubMed:2996781). EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency (PubMed:24067969, PubMed:2996781, PubMed:31142669, PubMed:8551585). EBNA1 binding to DS recruits the host origin recognition complex (ORC) (PubMed:12953058). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes (PubMed:11172042, PubMed:15479791, PubMed:24067969). Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes (PubMed:33482082). Counteracts the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival (PubMed:15808506). Induces degradation of host PML through the ubiquitin-proteasome system, which promotes lytic reactivation and may impair the host cell DNA repair (PubMed:18833293). Increases the association of CK2 with PML proteins which increases the phosphorylation of PML proteins by CK2, triggering the polyubiquitylation and degradation of PML (PubMed:20719947). Displays inhibitory effects on a SUMO2-modified complex that includes STUB1, KAP1 and USP7 (PubMed:32176739). This inhibitory effect possibly participates to the maintenance of latency linked to PML silencing (PubMed:32176739)

Subcellular Location

Host nucleus

Subunit

Homodimer (PubMed:19521517, PubMed:9878348). Dimers can assemble into higher-order oligomers like a homohexamer (PubMed:28701406). Binding to the DS element involves 2 dimers of EBNA1 (PubMed:14506283, PubMed:15808506, PubMed:18833293, PubMed:31142669). Interacts with human USP7; this interaction is independent and simultaneous to EBNA1 interaction with CSNK2B as well as necessary for PML nuclear bodies disruption by EBNA1 (PubMed:14506283, PubMed:15808506, PubMed:18833293, PubMed:20719947, PubMed:24216761). Interacts with host CSNK2B (via KSSR motif); the interaction requires phosphorylation of EBNA1, is independent and simultaneous to EBNA1 interaction with USP7 as well as necessary for PML nuclear bodies disruption by EBNA1. EBNA1, USP7 and CSNK2B form a ternary complex. EBNA1, USP7 and CSNK2B form a ternary complex (PubMed:20719947, PubMed:24216761). Interacts with human EBP2; it is not clear if this interaction is linked with the ability of EBNA1 to associate with host mitotic chromosomes (PubMed:10074103, PubMed:15479791). Interacts with BGLF4; this interaction facilitates the switch from latent to lytic DNA replication by down-regulating EBNA1 replication function (PubMed:19244323). Interacts with human PAX5; this interaction promotes EBNA1-dependent transcription (PubMed:31941781). Interacts with host KPNA1/Importin subunit alpha-5; this interaction allows the nuclear import of EBNA1 (PubMed:10612665). Interacts with host KPNA2/Importin subunit alpha-1; this interaction allows the nuclear import of EBNA1 (PubMed:10612665, PubMed:28104399, PubMed:9020106). Interacts with host C1QBP/P32 (PubMed:9299613). Interacts with host BIRC5/Survivin; this interaction is probably important for EBV episome maintenance in Burkitt's lymphoma host cells (PubMed:28077791)

Gene: EBNA1  |  Organism: Epstein-Barr virus (strain B95-8)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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