Recombinant Staphylococcus aureus Enterotoxin type A (entA), Staphylococcus aureus, Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 31.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Enterotoxin type A (entA)

Recombinant Staphylococcus aureus Enterotoxin type A (entA) — Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. Purity >90%.

SKU: BCRECP-002612 Category:
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Product Specifications

Product SkuBCRECP-002612
Product DescriptionRecombinant Staphylococcus aureus Enterotoxin type A (entA) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 25-257aa. [Accession | P0A0L2].
Uniprot No.P0A0L2
Gene NamesentA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region25-257aa
SpeciesStaphylococcus aureus
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight31.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, waves of cellular activation, cytokine production, and migration into the lung tissue and airways occur via alphabeta T-cells.

Product Specifications

Recombinant Staphylococcus aureus Enterotoxin type A (entA) — Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules (PubMed:12467569, PubMed:2658055). In turn, waves of cellular activation, cytokine production, and migration into the lung tissue and airways occur via alphabeta T-cells (PubMed:23321986). Also causes the intoxication staphylococcal food poisoning syndrome. The illness is characterized by high fever, hypotension, diarrhea, shock, and in some cases death (Probable)

Subcellular Location

Secreted

Subunit

Monomer. Interacts with MHC class II molecules alpha/HLA-DRB1 and beta/HLA-DRA chains (PubMed:11847286, PubMed:12467569, PubMed:2658055). The interaction with MHC-II molecules occurs at both zinc-dependent and zinc-independent sites (PubMed:12467569, PubMed:2658055). Interacts with T-cell receptor beta variable 7-9/TRBV7-9 (PubMed:27180909)

Gene: entA  |  Organism: Staphylococcus aureus  |  Synonyms: SEA
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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