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Recombinant Staphylococcus aureus Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (fabI)

Recombinant Staphylococcus aureus Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (fabI) — Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Purity >90%.

SKU: BCRECP-002693 Categories: ,

Product Specifications

Product SkuBCRECP-002693
Product DescriptionRecombinant Staphylococcus aureus Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (fabI) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-256aa. [Accession | Q6GI75].
Uniprot No.Q6GI75
Gene NamesfabI
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-256aa
SpeciesStaphylococcus aureus (strain MRSA252)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight44.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism.

Product Specifications

Recombinant Staphylococcus aureus Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (fabI) — Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity)

Subunit

Homotetramer

Pathway

Lipid metabolism; fatty acid biosynthesis

Gene: fabI  |  Organism: Staphylococcus aureus (strain MRSA252)  |  Synonyms: NADPH-dependent enoyl-ACP reductase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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