Recombinant Mouse F-box only protein 32 (Fbxo32), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 48.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse F-box only protein 32 (Fbxo32)

Recombinant Mouse F-box only protein 32 (Fbxo32) — Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Purity >85%.

SKU: BCRECP-002714 Category:
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Product Specifications

Product SkuBCRECP-002714
Product DescriptionRecombinant Mouse F-box only protein 32 (Fbxo32) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-355aa. [Accession | Q9CPU7].
Uniprot No.Q9CPU7
Gene NamesFbxo32
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-355aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight48.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy.

Product Specifications

Recombinant Mouse F-box only protein 32 (Fbxo32) — Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. Negatively regulates macrophage efferocytosis by promoting the ubiquitination of the transcription factor KLF4, suppressing the receptor tyrosine kinase MERTK transcription (PubMed:39986490). Regulates LPS-induced apoptosis and mitochondrial dysfunction through ANXA1 ubiquitination and degradation (PubMed:24789905). Mediates cyclin D1 protein stabilization via 'Lys-27'-linked ubiquitination (By similarity)

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Specifically expressed in cardiac and skeletal muscle

Subunit

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32. Interacts with KLF4; this interaction promotes KLF4 ubiquitination and degradation (PubMed:39986490). Negatively regulates macrophage efferocytosis by promoting the ubiquitination of the transcription factor KLF4, suppressing the receptor tyrosine kinase MERTK transcription (PubMed:39986490)

Pathway

Protein modification; protein ubiquitination

Gene: Fbxo32  |  Organism: Mus musculus  |  Synonyms: Atrogin-1; Muscle atrophy F-box protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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