Recombinant Mouse Fibrinogen-like protein 1 (Fgl1), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 40.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Fibrinogen-like protein 1 (Fgl1)

Recombinant Mouse Fibrinogen-like protein 1 (Fgl1) — Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a ligand of LAG3. Purity >90%.

SKU: BCRECP-002733 Category:
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Product Specifications

Product SkuBCRECP-002733
Product DescriptionRecombinant Mouse Fibrinogen-like protein 1 (Fgl1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 23-314aa. [Accession | Q71KU9].
Uniprot No.Q71KU9
Gene NamesFgl1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region23-314aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight40.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a ligand of LAG3. LAG3-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation. Binds LAG3 independently from MHC class II (MHC-II).

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Mainly expressed in liver. Also expressed in brown adipose tissue

Product Specifications

Recombinant Mouse Fibrinogen-like protein 1 (Fgl1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 23-314aa, with N-terminal 6xHis-tagged tag, molecular weight 40.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a ligand of LAG3 (PubMed:30580966). LAG3-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation (PubMed:30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed:30580966). Secreted by, and promotes growth of, hepatocytes (By similarity)

Subcellular Location

Secreted

Tissue Specificity

Mainly expressed in liver (PubMed:12528893, PubMed:23483972). Also expressed in brown adipose tissue (PubMed:23483972)

Subunit

Homodimer (By similarity). Interacts (via the Fibrinogen C-terminal domain) with LAG3 (via Ig-like domains 1 and 2) (PubMed:30580966)

Gene: Fgl1  |  Organism: Mus musculus  |  Synonyms: Fibrinogen-related protein 1; Liver fibrinogen-related protein-1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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