Recombinant Bordetella pertussis Serotype 3 fimbrial subunit (fim3), Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), E.coli, N-terminal 6xHis-tagged, 23.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bordetella pertussis Serotype 3 fimbrial subunit (fim3)

Recombinant Bordetella pertussis Serotype 3 fimbrial subunit (fim3) — Bordetella pertussis is the causative agent of whooping cough. Purity >85%.

SKU: BCRECP-002741 Category:
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Product Specifications

Product SkuBCRECP-002741
Product DescriptionRecombinant Bordetella pertussis Serotype 3 fimbrial subunit (fim3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 26-204aa. [Accession | P17835].
Uniprot No.P17835
Gene Namesfim3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region26-204aa
SpeciesBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Tag InfoN-terminal 6xHis-tagged
Molecular weight23.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Bordetella pertussis is the causative agent of whooping cough. An essential step in the disease process is the attachment of the bacteria to the ciliated epithelium of the respiratory tract, enabling the organism to resist normal host-clearance mechanisms. It is unclear which bacterial cell surface component are responsible for adherence but the fimbriae of B.pertussis are prime candidates for being involved in this process.

Biological Context

Subcellular Location: Fimbrium

Product Specifications

Recombinant Bordetella pertussis Serotype 3 fimbrial subunit (fim3) is a recombinant protein from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251), expressed in E.coli, covering amino acids 26-204aa, with N-terminal 6xHis-tagged tag, molecular weight 23.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Bordetella pertussis is the causative agent of whooping cough. An essential step in the disease process is the attachment of the bacteria to the ciliated epithelium of the respiratory tract, enabling the organism to resist normal host-clearance mechanisms. It is unclear which bacterial cell surface component are responsible for adherence but the fimbriae of B.pertussis are prime candidates for being involved in this process

Subcellular Location

Fimbrium

Gene: fim3  |  Organism: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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