Recombinant Serratia marcescens Hemophore HasA (hasA), Serratia marcescens, E.coli, N-terminal 6xHis-SUMO-tagged, 35.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Serratia marcescens Hemophore HasA (hasA)

Recombinant Serratia marcescens Hemophore HasA (hasA) — Can bind free heme and also acquire it from hemoglobin. Purity >90%.

SKU: BCRECP-002973 Category:
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Product Specifications

Product SkuBCRECP-002973
Product DescriptionRecombinant Serratia marcescens Hemophore HasA (hasA) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-188aa. [Accession | Q54450].
Uniprot No.Q54450
Gene NameshasA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-188aa
SpeciesSerratia marcescens
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight35.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Can bind free heme and also acquire it from hemoglobin. Conveys heme from hemoglobin to the HasR receptor which releases it into the bacterium. HasR alone can take up heme but the synergy between HasA and HasR increases heme uptake 100-fold.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Serratia marcescens Hemophore HasA (hasA) is a recombinant protein from Serratia marcescens, expressed in E.coli, covering amino acids 1-188aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 35.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Can bind free heme and also acquire it from hemoglobin. Conveys heme from hemoglobin to the HasR receptor which releases it into the bacterium. HasR alone can take up heme but the synergy between HasA and HasR increases heme uptake 100-fold

Subcellular Location

Secreted

Subunit

Monomer

Gene: hasA  |  Organism: Serratia marcescens  |  Synonyms: Heme acquisition system protein A

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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