Recombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21), Arabidopsis thaliana (Mouse-ear cress), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21)

Recombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21) — recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli. Purity >90%.

SKU: BCRECP-003025 Category:
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Product Specifications

Product SkuBCRECP-003025
Product DescriptionRecombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 44-227aa. [Accession | P31170].
Uniprot No.P31170
Gene NamesHSP21
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region44-227aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Chaperone protein required for seedling and chloroplast development under heat stress, probably by maintaining plastid-encoded RNA polymerase (PEP)-dependent transcription.

Biological Context

Subcellular Location: Plastid, chloroplast stroma, chloroplast nucleoid
Tissue Specificity: Confined to pollen grains of budding flowers

Product Specifications

Recombinant Arabidopsis thaliana Heat shock protein 21, chloroplastic (HSP21) is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 44-227aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Chaperone protein required for seedling and chloroplast development under heat stress, probably by maintaining plastid-encoded RNA polymerase (PEP)-dependent transcription

Subcellular Location

Plastid, chloroplast stroma, chloroplast nucleoid

Tissue Specificity

Confined to pollen grains of budding flowers

Subunit

Forms oligomeric structures: dodecameric arrangement of two trimer- of-dimer disks stabilized by the C-terminal tails, possibly through tail-to-tail interactions between the disks (PubMed:28325834). Interacts with PTAC5; the formed complex associates with the plastid-encoded RNA polymerase (PEP) complex not only during transcription initiation, but also during elongation and termination, and with a stronger efficiency in illuminated chloroplasts. Binds to promoter regions of PEP-dependent genes (PubMed:23922206)

Gene: HSP21  |  Organism: Arabidopsis thaliana  |  Synonyms: 25.3 kDa heat shock protein, chloroplastic
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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