Recombinant Mouse Zinc finger protein Helios (Ikzf2), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 66.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Zinc finger protein Helios (Ikzf2)

Recombinant Mouse Zinc finger protein Helios (Ikzf2) — Transcription factor, which stabilizes the noninflammatory phenotype of regulatory T cells (Tregs). Purity >85%.

SKU: BCRECP-003102 Category:
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Product Specifications

Product SkuBCRECP-003102
Product DescriptionRecombinant Mouse Zinc finger protein Helios (Ikzf2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-526aa. [Accession | P81183].
Uniprot No.P81183
Gene NamesIkzf2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-526aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight66.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Transcription factor, which stabilizes the noninflammatory phenotype of regulatory T cells (Tregs). Therefore plays a crucial role in self-tolerance. May possibly act via STAT5-mediated signaling.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Expressed in outer hair cells (OHC) of the organ of Corti. Abundant in thymus, low expression in bone marrow and brain and no detectable expression in spleen, liver, kidney or muscle. Expressed in T-cells. In Tregs, expressed in 2 specific lineages: FoxP3(+)CD4(+) and Ly49(+)CD8(+)

Product Specifications

Recombinant Mouse Zinc finger protein Helios (Ikzf2) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-526aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 66.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Transcription factor, which stabilizes the noninflammatory phenotype of regulatory T cells (Tregs). Therefore plays a crucial role in self-tolerance (PubMed:26472910). May possibly act via STAT5-mediated signaling (PubMed:26472910). Binds IL2 promoter and represses its transcription, possibly by epigenetic silencing, inducing deacetylation of core histones in the IL2 promoter (PubMed:23275607). In Tregs, required for FOXP3 recruitment to the IL2 promoter (PubMed:23275607). Required for outer hair cells maturation and, consequently, for hearing (PubMed:30464345)

Subcellular Location

Nucleus

Tissue Specificity

Expressed in outer hair cells (OHC) of the organ of Corti (PubMed:30464345). Abundant in thymus, low expression in bone marrow and brain and no detectable expression in spleen, liver, kidney or muscle. Expressed in T-cells (PubMed:9512513). In Tregs, expressed in 2 specific lineages: FoxP3(+)CD4(+) and Ly49(+)CD8(+) (PubMed:23275607, PubMed:26472910)

Subunit

Can form homodimers (PubMed:30464345). Interacts with IKZF1 and IKZF3 (By similarity). Interacts with IKZF4 (By similarity). Interacts with IKZF5 (By similarity). Interacts with HDAC1 (By similarity)

Gene: Ikzf2  |  Organism: Mus musculus  |  Synonyms: Ikaros family zinc finger protein 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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