Recombinant Salmonella typhimurium Invasion protein InvE (invE), Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720), E.coli, C-terminal 6xHis-tagged, 49.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Salmonella typhimurium Invasion protein InvE (invE)

Recombinant Salmonella typhimurium Invasion protein InvE (invE) — Involved in the triggering of intracellular events that lead to microbial internalization. Purity >85%.

SKU: BCRECP-003183 Category:
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Product Specifications

Product SkuBCRECP-003183
Product DescriptionRecombinant Salmonella typhimurium Invasion protein InvE (invE) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-372aa. [Accession | P35671].
Uniprot No.P35671
Gene NamesinvE
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-372aa
SpeciesSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Tag InfoC-terminal 6xHis-tagged
Molecular weight49.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in the triggering of intracellular events that lead to microbial internalization. These events include increase in calcium level, redistribution of actin microfilaments, and changes in the normal structure of the microvilli. Encoded within the type III secretion system (SPI-1 T3SS), it is essential for the translocation of protein effectors into host cells.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Salmonella typhimurium Invasion protein InvE (invE) is a recombinant protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720), expressed in E.coli, covering amino acids 1-372aa, with C-terminal 6xHis-tagged tag, molecular weight 49.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in the triggering of intracellular events that lead to microbial internalization. These events include increase in calcium level, redistribution of actin microfilaments, and changes in the normal structure of the microvilli. Encoded within the type III secretion system (SPI-1 T3SS), it is essential for the translocation of protein effectors into host cells. Forms a complex with SipB and SipC in the presence of their chaperone SicA. Positively regulates the secretion of SPI-1 T3SS effector proteins SipB, SipC and SipD and negatively influences the secretion of SipA, SopA and SptP

Subcellular Location

Cell membrane (Peripheral membrane protein)

Gene: invE  |  Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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