Recombinant Glycine max Leghemoglobin A (LBA), Glycine max (Soybean) (Glycine hispida), Yeast, C-terminal 6xHis-tagged, 16.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Glycine max Leghemoglobin A (LBA)

Recombinant Glycine max Leghemoglobin A (LBA) — Leghemoglobin that reversibly binds oxygen O(2) through a pentacoordinated heme iron. Purity >90%.

SKU: BCRECP-003242 Category:
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Product Specifications

Product SkuBCRECP-003242
Product DescriptionRecombinant Glycine max Leghemoglobin A (LBA) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 2-144aa. [Accession | P02238].
Uniprot No.P02238
Gene NamesLBA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-144aa
SpeciesGlycine max (Soybean) (Glycine hispida)
Tag InfoC-terminal 6xHis-tagged
Molecular weight16.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Leghemoglobin that reversibly binds oxygen O(2) through a pentacoordinated heme iron. In root nodules, facilitates the diffusion of oxygen to the bacteroids while preventing the bacterial nitrogenase from being inactivated by buffering dioxygen, nitric oxide and carbon monoxide, and promoting the formation of reactive oxygen species (ROS, e.g. H(2)O(2)).

Biological Context

Subcellular Location: Cytoplasm, cytosol; Nucleus
Tissue Specificity: Specifically expressed in root nodules

Product Specifications

Recombinant Glycine max Leghemoglobin A (LBA) is a recombinant protein from Glycine max (Soybean) (Glycine hispida), expressed in Yeast, covering amino acids 2-144aa, with C-terminal 6xHis-tagged tag, molecular weight 16.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Leghemoglobin that reversibly binds oxygen O(2) through a pentacoordinated heme iron (PubMed:29100196). In root nodules, facilitates the diffusion of oxygen to the bacteroids while preventing the bacterial nitrogenase from being inactivated by buffering dioxygen, nitric oxide and carbon monoxide, and promoting the formation of reactive oxygen species (ROS, e.g. H(2)O(2)) (PubMed:17540516, PubMed:22308405, PubMed:29701804, PubMed:32297921). This role is essential for symbiotic nitrogen fixation (SNF) (PubMed:17540516, PubMed:32297921)

Subcellular Location

Cytoplasm, cytosol; Nucleus

Tissue Specificity

Specifically expressed in root nodules

Subunit

Monomer

Gene: LB3  |  Organism: Glycine max  |  Synonyms: Hemoglobin 2-4; Leghemoglobin alpha; Nodulin-2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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