Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 48.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1)

Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1) — Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Purity >90%.

SKU: BCRECP-003268 Category:
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Product Specifications

Product SkuBCRECP-003268
Product DescriptionRecombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-357aa. [Accession | P11260].
Uniprot No.P11260
Gene NamesLire1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-357aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight48.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.

Biological Context

Subcellular Location: Nucleus, nucleolus; Cytoplasm; Cytoplasm, Cytoplasmic ribonucleoprotein granule; Cytoplasm, Stress granule
Tissue Specificity: Expressed in meiotic spermatocytes and in the cerebellum (at protein level)

Product Specifications

Recombinant Mouse LINE-1 retrotransposable element ORF1 protein (Lire1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-357aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 48.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded

Subcellular Location

Nucleus, nucleolus; Cytoplasm; Cytoplasm, Cytoplasmic ribonucleoprotein granule; Cytoplasm, Stress granule

Tissue Specificity

Expressed in meiotic spermatocytes and in the cerebellum (at protein level)

Subunit

Homotrimer (via coiled coil domain). May also form larger homooligomers. Interacts with Tex19.1 and UBR2 (PubMed:28806172). Interacts with MOV10 (PubMed:28662698)

Gene: Lire1  |  Organism: Mus musculus  |  Synonyms: LINE retrotransposable element 1; LINE1 retrotransposable element 1; Transposase element L1Md-A101/L1Md-A102/L1Md-A2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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