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Mouse anti-Bacteriophage M13 phage M13 III Monoclonal Antibody

Mouse anti-Bacteriophage M13 phage M13 III Monoclonal Antibody — Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process.

SKU: BCREC-000597MA Category:
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Product Specifications

Uniprot No.P69168
Target NamesIII
Species ReactivityMouse
ImmunogenRecombinant Enterobacteria phage M13 Attachment protein (19-424AA)
Immunogen SpeciesEnterobacteria phage M13
ConjugateNon-conjugated
IsotypeIgG1
ClonalityMonoclonal
ApplicationsELISA, WB
BufferPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Function

Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Attachment of the phage causes pilus retraction bringing the viral particle into close proximity of the host cell inner membrane.

Product Specifications

Mouse anti-Bacteriophage M13 phage M13 III Monoclonal Antibody is a recombinant protein. Suitable for ELISA and Western Blot applications.

Catalog number: BCREC-000597MA.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Attachment of the phage causes pilus retraction bringing the viral particle into close proximity of the host cell inner membrane. Binding to the host pilus initiates a change in the G3P conformation, allowing subsequent interaction with the host entry receptors TolA, TolQ and TolR and penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain

Gene: III  |  Organism: Enterobacteria phage M13

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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