Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-Myc-tagged, 43.9 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active)

Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active) — Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. Purity >90%.

SKU: BCACP-000037 Category:
Online Inquiry →
Product SKUBCACP-000037
Product DescriptionRecombinant Human B- and T-lymphocyte attenuator (BTLA) protein is expressed from Mammalian cell with C-terminal hFc1-Myc-tagged. It contains 31-150aa. [Accession | Q7Z6A9].
Uniprot No.Q7Z6A9
Gene NamesBTLA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region31-150aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-Myc-tagged
Molecular weight43.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14 , the EC50 is 137.8-233.4 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(B- and T-lymphocyte-associated protein)(CD272)

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Human B- and T-lymphocyte attenuator (BTLA), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 31-150aa, with C-terminal hFc1-Myc-tagged tag, molecular weight 43.9 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006). Interacts with TNFRSF14/HVEM (via cysteine-rich domain 1) (PubMed:15568026, PubMed:18193050, PubMed:19915044)

Gene: BTLA  |  Organism: Homo sapiens  |  Synonyms: B- and T-lymphocyte-associated protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top