Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 48.0 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
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Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active)

Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) — Receptor for TNFSF5/CD40LG. Purity >95%.

SKU: BCACP-000096 Categories: , ,
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Product SKUBCACP-000096
Product DescriptionRecombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40) protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 21-193aa. [Accession | P25942].
Uniprot No.P25942
Gene NamesCD40
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region21-193aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight48.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 μg/ml can bind TNFRSF13C, the EC50 is 9.943-15.72 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C at 2 μg/ml can bind Biotinylated human TNFSF13B , the EC50 is 0.2699-0.5613 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCD40; TNFRSF5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD antigen CD40

Function

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: B-cells and in primary carcinomas
Disease Association: Immunodeficiency with hyper-IgM 3 (HIGM3) : A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 21-193aa, with C-terminal hFc1-tagged tag, molecular weight 48.0 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity)

Subcellular Location

Secreted

Disease Association

Immunodeficiency with hyper-IgM 3 (HIGM3) : A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

B-cells and in primary carcinomas

Subunit

Monomer and homodimer. The variant form found in the bladder carcinoma cell line Hu549 does not form homodimers. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts with TRAF6 and MAP3K8; the interaction is required for ERK activation

Gene: CD40  |  Organism: Homo sapiens  |  Synonyms: B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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