Recombinant Human Heat-stable enterotoxin receptor (GUCY2C), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 74.9 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Heat-stable enterotoxin receptor (GUCY2C), partial (Active)

Recombinant Human Heat-stable enterotoxin receptor (GUCY2C), partial (Active) — Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP. Purity >90%.

SKU: BCACP-000128 Category:
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Product SKUBCACP-000128
Product DescriptionRecombinant Human Heat-stable enterotoxin receptor (GUCY2C) protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 24-430aa. [Accession | P25092].
Uniprot No.P25092
Gene NamesGUCY2C
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region24-430aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight74.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized N ) at 2 μg/ml can bind N Mouse Monoclonal Antibody, the EC50 of N Mouse Monoclonal Antibody is 11.40-19.77 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized N ) at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody is 63.78-113.6 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(STA receptor)(hSTAR)(Guanylyl cyclase C)(GC-C)(Intestinal guanylate cyclase)

Function

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP. Receptor for the E.coli heat-stable enterotoxin; E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GUCY2C. Also activated by the endogenous peptides guanylin and uroguanylin.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Endoplasmic reticulum membrane (Single-pass type I membrane protein)
Disease Association: Diarrhea 6 (DIAR6) : A relatively mild, early-onset chronic diarrhea that may be associated with increased susceptibility to inflammatory bowel disease, small bowel obstruction, and esophagitis. [The disease is caused by variants affecting the gene represented in this entry] | Meconium ileus (MECIL) : A condition characterized by intestinal obstruction due to inspissated meconium in the distal ileum and cecum, which develops in utero and presents shortly after birth as a failure to pass meconium. Meconium ileus is a known clinical manifestation of cystic fibrosis. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Heat-stable enterotoxin receptor (GUCY2C), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 24-430aa, with C-terminal hFc1-tagged tag, molecular weight 74.9 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP (PubMed:11950846, PubMed:1718270, PubMed:22436048, PubMed:22521417, PubMed:23269669). Receptor for the E.coli heat-stable enterotoxin; E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GUCY2C (PubMed:1680854, PubMed:1718270). Also activated by the endogenous peptides guanylin and uroguanylin (PubMed:8381596)

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Endoplasmic reticulum membrane (Single-pass type I membrane protein)

Disease Association

Diarrhea 6 (DIAR6) : A relatively mild, early-onset chronic diarrhea that may be associated with increased susceptibility to inflammatory bowel disease, small bowel obstruction, and esophagitis. [The disease is caused by variants affecting the gene represented in this entry] | Meconium ileus (MECIL) : A condition characterized by intestinal obstruction due to inspissated meconium in the distal ileum and cecum, which develops in utero and presents shortly after birth as a failure to pass meconium. Meconium ileus is a known clinical manifestation of cystic fibrosis. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homotrimer (PubMed:11123935). Interacts via its C-terminal region with NHERF4 (PubMed:11950846). Interacts with the lectin chaperone VIP36 (PubMed:23269669)

Gene: GUCY2C  |  Organism: Homo sapiens  |  Synonyms: Heat-stable enterotoxin receptor; Intestinal guanylate cyclase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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