Recombinant Human Tumor necrosis factor ligand superfamily member 9 (TNFSF9), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal hFc-Myc-tagged, 48.0 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Tumor necrosis factor ligand superfamily member 9 (TNFSF9), partial (Active)

Recombinant Human Tumor necrosis factor ligand superfamily member 9 (TNFSF9), partial (Active) — Cytokine that binds to TNFRSF9. Purity >90%.

SKU: BCACP-000149 Categories: ,
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Product SKUBCACP-000149
Product DescriptionRecombinant Human Tumor necrosis factor ligand superfamily member 9 (TNFSF9) protein is expressed from Mammalian cell with N-terminal hFc-Myc-tagged. It contains 71-254aa. [Accession | P41273].
Uniprot No.P41273
Gene NamesTNFSF9
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region71-254aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal hFc-Myc-tagged
Molecular weight48.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 μg/mL can bind anti-NECTIN4 antibody（CSB-RA822274A0HU）（enfortumab vedotin-like）, the EC50 is 0.6029-0.7837 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(4-1BB ligand) (4-1BBL)

Function

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD).

Biological Context

Subcellular Location: Membrane (Single-pass type II membrane protein)
Tissue Specificity: Expressed in brain, placenta, lung, skeletal muscle and kidney

Product Specifications

Recombinant Human Tumor necrosis factor ligand superfamily member 9 (TNFSF9), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 71-254aa, with N-terminal hFc-Myc-tagged tag, molecular weight 48.0 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages

Subcellular Location

Membrane (Single-pass type II membrane protein)

Tissue Specificity

Expressed in brain, placenta, lung, skeletal muscle and kidney

Subunit

Homotrimer

Gene: TNFSF9  |  Organism: Homo sapiens  |  Synonyms: 4-1BB ligand

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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