Recombinant Human Prominin-1 (PROM1)-VLPs (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions), 96.7 kDa
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Recombinant Human Prominin-1 (PROM1)-VLPs (Active)

Recombinant Human Prominin-1 (PROM1)-VLPs (Active) — May play a role in cell differentiation, proliferation and apoptosis.

SKU: BCACP-000214 Categories: ,
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Product SKUBCACP-000214
Product DescriptionRecombinant Human Prominin-1 (PROM1)-VLPs protein is expressed from Mammalian cell with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions). It contains 20-865aa. [Accession | O43490].
Uniprot No.O43490
Gene NamesPROM1
Expression SystemMammalian cell
Expression Region20-865aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Molecular weight96.7 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human DSG3 at 2 μg/mL can bind Anti-DSG3 recombinant antibody , the EC50 is 4.552-5.720 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesProminin-1 (Antigen AC133)(Prominin-like protein 1)(CD antigen CD133)

Function

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis.

Product Specifications

Recombinant Human Prominin-1 (PROM1)-VLPs (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 20-865aa, with C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) tag, molecular weight 96.7 kDa. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439)

Gene: PROM1  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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