| Product SKU | BCACP-000276 |
| Product Description | Recombinant Human Endoplasmic reticulum chaperone BiP (HSPA5) protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 19-654aa. [Accession | P11021]. |
| Uniprot No. | P11021 |
| Gene Names | HSPA5 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | Yeast |
| Expression Region | 19-654aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 72.0 kDa |
| Activity | Loaded Anti-Human GPC3 Antibody on 96-Flat plate, can bind Human GPC3(S359F), with an affinity constant of 6.32 nM as determined in BLI assay (Gator Prime). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Endoplasmic reticulum chaperone BiP; EC 3.6.4.10; 78 kDa glucose-regulated protein; GRP-78; Binding-immunoglobulin protein; BiP; Heat shock protein 70 family protein 5; HSP70 family protein 5; Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
