Recombinant Macaca fascicularis Tissue factor (F3), partial (Active), Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), Mammalian cell, C-terminal 10xHis-tagged, 26.4 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Macaca fascicularis Tissue factor (F3), partial (Active)

Recombinant Macaca fascicularis Tissue factor (F3), partial (Active) — Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. Purity >95%.

SKU: BCACP-000445 Category:
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Product SKUBCACP-000445
Product DescriptionRecombinant Cynomolgus monkey Tissue factor (F3) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 33-252aa. [Accession | A0A2K5VX02].
Uniprot No.A0A2K5VX02
Gene NamesF3
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region33-252aa
SpeciesMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Tag InfoC-terminal 10xHis-tagged
Molecular weight26.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human PTPRC protein at 2 μg/mL can bind Anti-PTPRC recombinant antibody . The EC50 is 25.15-29.54 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesTissue factor; Coagulation factor III; F3

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Product Specifications

Recombinant Macaca fascicularis Tissue factor (F3), partial (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 33-252aa, with C-terminal 10xHis-tagged tag, molecular weight 26.4 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade

Organism: Macaca fascicularis

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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