Recombinant Mouse Tumor necrosis factor (Tnf), partial (Active) — recombinant protein, expressed in Yeast. Purity >95%.
| Product SKU | BCACP-000528 |
| Product Description | Recombinant Mouse Tumor necrosis factor (Tnf) protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 80-235aa. [Accession | P06804]. |
| Uniprot No. | P06804 |
| Gene Names | Tnf |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC. |
| Expression System | Yeast |
| Expression Region | 80-235aa |
| Species | Mus musculus (Mouse) |
| Tag Info | N-terminal 6xHis-tagged |
| Molecular weight | 19.3 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF17 protein at 2 μg/mL can bind Anti-TNFRSF17 recombinant antibody . The EC50 is 5.652-7.215 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF17 protein at 2 μg/mL can bind Anti-TNFRSF17 recombinant antibody . The EC50 is 2.296-2.754ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF17 protein at 2 μg/mL can bind Anti-TNFRSF17 recombinant antibody . The EC50 is 14.97-19.19 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF17 protein at 2 μg/mL can bind Human TNFSF13 protein . The EC50 is 4.217-5.674 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF17 protein at 2 μg/mL can bind Human TNFSF13B protein . The EC50 is 4.346-5.534 ng/mL. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2 (TNF-a); Tnf; Tnfa, Tnfsf2 |
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Recombinant Mouse Tumor necrosis factor (Tnf), partial (Active) is a recombinant protein, expressed in Yeast, covering amino acids 80-235aa, with N-terminal 6xHis-tagged tag, molecular weight 19.3 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications. Explore more TNF Family proteins →
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
