| Product SKU | BCACP-000637 |
| Product Description | Recombinant Human Coagulation factor IX (F9) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 29-461aa. [Accession | P00740]. |
| Uniprot No. | P00740 |
| Gene Names | F9 |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC. |
| Expression System | Mammalian cell |
| Expression Region | 29-461aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 10xHis-tagged |
| Molecular weight | 50.1 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 2.428-2.771 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GPC3 at 2 μg/mL can bind Anti-GPC3 recombinant antibody . The EC50 is 4.451-5.233 ng/mL.;GPC3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis GPC3 with an affinity constant of 0.392 nM as detected by MetaSPR Assay (WeSPRTM 200).;GPC3 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Macaca fascicularis GPC3 with an affinity constant of 0.228 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Coagulation factor IX; EC 3.4.21.22; Christmas factor; Plasma thromboplastin component; PTC [Cleaved into: Coagulation factor IXa light chain; Coagulation factor IXa heavy chain] |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
