Recombinant Human Interleukin-7 protein (IL7), Homo sapiens (Human), E.Coli, Tag-Free, 17.4 kDa, purity >>97% as determined by SDS-PAGE.
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Recombinant Human Interleukin-7 protein (IL7)

Recombinant Human Interleukin-7 protein (IL7) — Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maint… Purity >97%.

SKU: BCACP-000679 Categories: ,
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Product SKUBCACP-000679
Product DescriptionRecombinant Human Interleukin-7 protein (IL7) protein is expressed from E.Coli with Tag-Free. It contains 26-177aa. [Accession | P13232].
Uniprot No.P13232
Gene NamesIL7
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region26-177aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight17.4 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine B9-11 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIL7; Interleukin-7; IL-7

Function

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.

Biological Context

Subcellular Location: Secreted
Disease Association: Immunodeficiency 130 with HPV-related verrucosis (IMD130) : An autosomal recessive immunologic disorder characterized by susceptibility to human papillomavirus (HPV) infections that lead to the development of warts and verrucous or plaque-like skin lesions. There is an increased risk of skin malignancy. Some patients may also suffer from mycobacterial, herpes simplex virus (HSV), or fungal infections. Immunologic workup shows T-cell lymphopenia, particularly affecting CD4+ T cells. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Interleukin-7 protein (IL7) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 26-177aa, with Tag-Free tag, molecular weight 17.4 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed:25870237, PubMed:7527823). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG (PubMed:8128231). Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5 (PubMed:18523275, PubMed:20974963)

Subcellular Location

Secreted

Disease Association

Immunodeficiency 130 with HPV-related verrucosis (IMD130) : An autosomal recessive immunologic disorder characterized by susceptibility to human papillomavirus (HPV) infections that lead to the development of warts and verrucous or plaque-like skin lesions. There is an increased risk of skin malignancy. Some patients may also suffer from mycobacterial, herpes simplex virus (HSV), or fungal infections. Immunologic workup shows T-cell lymphopenia, particularly affecting CD4+ T cells. [The disease may be caused by variants affecting the gene represented in this entry]

Subunit

Interacts with IL7R and CSF2RG

Gene: IL7  |  Organism: Homo sapiens
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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