Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2), Homo sapiens (Human), E.Coli, C-terminal 6xHis-tagged, 64.4 kDa, purity >>95% as determined by SDS-PAGE.
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Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2)

Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2) — recombinant protein from Homo sapiens (Human), expressed in E.Coli. Purity >95%.

SKU: BCACP-000848 Categories: ,
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Product SKUBCACP-000848
Product DescriptionRecombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2) protein is expressed from E.Coli with C-terminal 6xHis-tagged. It contains 19-584aa. [Accession | P23368].
Uniprot No.P23368
Gene NamesME2
Purity>95% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region19-584aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight64.4 kDa
ActivityFully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 3000 U/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesMalate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD(+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD(+) dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase

Function

NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.

Biological Context

Subcellular Location: Mitochondrion matrix

Product Specifications

Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 19-584aa, with C-terminal 6xHis-tagged tag, molecular weight 64.4 kDa, purity >95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate

Subcellular Location

Mitochondrion matrix

Subunit

Homotetramer

Gene: ME2  |  Organism: Homo sapiens  |  Synonyms: Malic enzyme 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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