Recombinant Human Thymosin beta-4 (TMSB4X) (Active), Homo sapiens (Human), E.Coli, Tag-Free, 4.9 kDa, purity >>97% as determined by SDS-PAGE.

Recombinant Human Thymosin beta-4 (TMSB4X) (Active)

Recombinant Human Thymosin beta-4 (TMSB4X) (Active) — Potent inhibitor of bone marrow derived stem cell differentiation. Purity >97%.

SKU: BCACP-000867 Category:
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Product SKUBCACP-000867
Product DescriptionRecombinant Human Thymosin beta-4 (TMSB4X) protein is expressed from E.Coli with Tag-Free. It contains 2-44aa. [Accession | P62328].
Uniprot No.P62328
Gene NamesTMSB4X
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region2-44aa
SpeciesHomo sapiens (Human)
Tag InfoTag-Free
Molecular weight4.9 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesFx; Hematopoietic system regulatory peptide; Prothymosin beta 4; PTMB 4; PTMB4; Seraspenide; T beta 4; T beta-4; TB4X; THYB 4; Thyb4; Thymosin beta 4; Thymosin beta 4 X chromosome; Thymosin beta 4 X linked; TMSB 4; TMSB4; Tmsb4x; TYB4_HUMAN

Function

Potent inhibitor of bone marrow derived stem cell differentiation. Acts by inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.

Biological Context

Subcellular Location: Cytoplasm, cytoskeleton
Tissue Specificity: Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells

Product Specifications

Recombinant Human Thymosin beta-4 (TMSB4X) (Active) is a recombinant protein from Homo sapiens (Human), expressed in E.Coli, covering amino acids 2-44aa, with Tag-Free tag, molecular weight 4.9 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Potent inhibitor of bone marrow derived stem cell differentiation (PubMed:7694679). Acts by inhibits the entry of hematopoietic pluripotent stem cells into the S-phase (By similarity)

Subcellular Location

Cytoplasm, cytoskeleton

Tissue Specificity

Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells

Subunit

Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (PubMed:23009842). Interacts with SERPINB1 (By similarity)

Gene: TMSB4X  |  Organism: Homo sapiens  |  Synonyms: Fx
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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