Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active), Mus musculus (Mouse), E.Coli, Tag-Free, 20.0 kDa, purity >>98% as determined by SDS­PAGE.

Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active)

Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active) — The dually lipidated desert hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Purity >98%.

SKU: BCACP-000877 Category:
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Product SKUBCACP-000877
Product DescriptionRecombinant Mouse Desert hedgehog protein (Dhh) protein is expressed from E.Coli with Tag-Free. It contains 24-198aa. [Accession | Q61488].
Uniprot No.Q61488
Gene NamesDhh
Purity>98% as determined by SDS­PAGE.
Expression SystemE.Coli
Expression Region24-198aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight20.0 kDa
ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesDhhDesert hedgehog protein; DHH; HHG-3) [Cleaved into: Desert hedgehog protein N-product; Desert hedgehog protein C-product]

Function

The dually lipidated desert hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes. In the absence of DHH, patched represses the constitutive signaling activity of SMO.

Biological Context

Subcellular Location: Secreted; Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in adult testes. Not expressed in limb buds

Product Specifications

Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 24-198aa, with Tag-Free tag, molecular weight 20.0 kDa, purity >98% as determined by SDS­PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The dually lipidated desert hedgehog protein N-product is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development (PubMed:40494346). Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes (PubMed:33063110, PubMed:40494346, PubMed:9811851). In the absence of DHH, patched represses the constitutive signaling activity of SMO (By similarity). DHH plays a key role in male gonad development (spermatogenesis) and the formation of Schwann cells (PubMed:10482238, PubMed:11090455, PubMed:8805249). Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules (PubMed:11090455, PubMed:8805249). In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube (PubMed:10482238). May induce motor neurons in lateral neural tube and may have a polarizing activity (By similarity). Involved in tissue regeneration: secreted from epithelial neuroendocrine cells and elicits a regenerative response from mesenchymal cells (PubMed:40494346). Functions in cell-cell mediated juxtacrine signaling (By similarity). Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism (PubMed:32151560). Promotes endothelium integrity (PubMed:33063110)

Subcellular Location

Secreted; Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in adult testes (PubMed:8805249). Not expressed in limb buds

Subunit

Multimer

Gene: Dhh  |  Organism: Mus musculus  |  Synonyms: HHG-3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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