Recombinant Human herpesvirus 8 type P Viral macrophage inflammatory protein 2 (VMI2), partial (Active), Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus), E.Coli, Tag-Free, 8.0 kDa, purity >>97% as determined by SDS-PAGE.
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Recombinant Human herpesvirus 8 type P Viral macrophage inflammatory protein 2 (VMI2), partial (Active)

Recombinant Human herpesvirus 8 type P Viral macrophage inflammatory protein 2 (VMI2), partial (Active) — Viral CC chemokine that binds to a broad spectrum of viral and human chemokine receptors and, depending on the receptor, acts either as an antagonist or an agonist. Purity >97%.

SKU: BCACP-000881 Categories: ,
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Product SKUBCACP-000881
Product DescriptionRecombinant Kaposi's sarcoma-associated herpesvirus Viral macrophage inflammatory protein 2 (VMI2) protein is expressed from E.Coli with Tag-Free. It contains 24-93aa. [Accession | Q98157].
Uniprot No.Q98157
Gene NamesORF
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region24-93aa
SpeciesHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Tag InfoTag-Free
Molecular weight8.0 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of >2.5x104 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesORF; K4; Viral macrophage inflammatory protein 2; Viral macrophage inflammatory protein II; vMIP-II; vMIP-1B

Function

Viral CC chemokine that binds to a broad spectrum of viral and human chemokine receptors and, depending on the receptor, acts either as an antagonist or an agonist. Capable of binding to chemokine receptors of the 4 families (CCR, CXCR, XCR, and CX3CR). Acts as an antagonist toward CCR1, CCR2, CCR5, and CCR10, competing with the endogenous chemokine binding and signaling.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Human herpesvirus 8 type P Viral macrophage inflammatory protein 2 (VMI2), partial (Active) is a recombinant protein from Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus), expressed in E.Coli, covering amino acids 24-93aa, with Tag-Free tag, molecular weight 8.0 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Viral CC chemokine that binds to a broad spectrum of viral and human chemokine receptors and, depending on the receptor, acts either as an antagonist or an agonist. Capable of binding to chemokine receptors of the 4 families (CCR, CXCR, XCR, and CX3CR). Acts as an antagonist toward CCR1, CCR2, CCR5, and CCR10, competing with the endogenous chemokine binding and signaling (PubMed:9287217). On the other hand, acts as an agonist of CCR3 and CCR8, triggering G protein-mediated intracellular calcium release and activation of the ERK and PI3K/AKT signaling pathways, or chemotaxis (PubMed:9323208, PubMed:9834206, PubMed:11500830). Thereby, participates in the skewing of the host immune system away from the Th1 cytotoxic response, undesirable for the virus-infected cells, toward a Th2 response, which is less effective against intracellular pathogens (PubMed:9834206, PubMed:11500830). CXCR7 acts as a scavenger receptor for vCCL2, regulating its availability and activity toward other host receptors (PubMed:27238288)

Subcellular Location

Secreted

Subunit

Monomer. Interacts with human chemokine receptor CXCR4 (PubMed:25612609). Interacts with host CCR3 (PubMed:9323208, PubMed:11500830). Interacts with host CCR8 (PubMed:9834206). Interacts with host CCR1, CCR2 and CCR5 (PubMed:9287217). Interacts with ACKR3/CXCR7; this interaction regulates vCCL2 availability and activity toward human receptors (PubMed:27238288)

Gene: vCCL2  |  Organism: Human herpesvirus 8 type P (isolate GK18)  |  Synonyms: Viral macrophage inflammatory protein II; vMIP-1B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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