Recombinant Human Tumor necrosis factor (TNF)，Partial (Active), Homo sapiens (Human), Mammalian cell, Tag free, 17.4 kDa, purity >95% as determined by SDS-PAGE.
,

Recombinant Human Tumor necrosis factor (TNF)，Partial (Active)

Recombinant Human Tumor necrosis factor (TNF)，Partial (Active) — recombinant protein from Homo sapiens (Human), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-001161 Categories: ,
Online Inquiry →
Product SKUBCACP-001161
Product DescriptionRecombinant Human Tumor necrosis factor (TNF)，Partial protein is expressed from Mammalian cell with Tag free. It contains 77-233aa. [Accession | P01375].
Uniprot No.P01375
Gene NamesTNF-α
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region77-233aa
SpeciesHomo sapiens (Human)
Tag InfoTag free
Molecular weight17.4 kDa
ActivityMeasured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Namesumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2

Function

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Biological Context

Subcellular Location: Secreted
Disease Association: Psoriatic arthritis (PSORAS) : An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Immunodeficiency 127 (IMD127) : An autosomal recessive immunologic disorder characterized by increased susceptibility to pulmonary infection with Mycobacterium tuberculosis. Affected individuals develop recurrent pulmonary tuberculosis, but have no adverse reaction to live BCG vaccination. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Tumor necrosis factor (TNF)，Partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 77-233aa, with Tag free tag, molecular weight 17.4 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells

Subcellular Location

Secreted

Disease Association

Psoriatic arthritis (PSORAS) : An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Immunodeficiency 127 (IMD127) : An autosomal recessive immunologic disorder characterized by increased susceptibility to pulmonary infection with Mycobacterium tuberculosis. Affected individuals develop recurrent pulmonary tuberculosis, but have no adverse reaction to live BCG vaccination. [The disease may be caused by variants affecting the gene represented in this entry]

Subunit

Homotrimer. Interacts with SPPL2B

Gene: TNF  |  Organism: Homo sapiens  |  Synonyms: Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top