Recombinant Human Hepatocyte growth factor (HGF) (Active), Homo sapiens (Human), Mammalian cell, C-terminal 6xHis-tagged, 79.7 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human Hepatocyte growth factor (HGF) (Active)

Recombinant Human Hepatocyte growth factor (HGF) (Active) — Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Purity >95%.

SKU: BCACP-001186 Categories: ,
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Product SKUBCACP-001186
Product DescriptionRecombinant Human Hepatocyte growth factor (HGF) protein is expressed from Mammalian cell with C-terminal 6xHis-tagged. It contains 32-728aa. [Accession | P14210].
Uniprot No.P14210
Gene NamesHGF
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region32-728aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight79.7 kDa
ActivityThe ED50 as determined in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells is 20-100 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesDFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor; Hepatocyte growth factor beta chain; Hepatocyte growth factor precursor; Hepatopoietin A; Hepatopoietin-A; Hgf; HGF_HUMAN; HGFB; HPTA; Lung fibroblast derived mitogen; OTTHUMP00000161349; OTTHUMP00000206710; OTTHUMP00000206711; OTTHUMP00000206712; OTTHUMP00000206713; OTTHUMP00000206730; Scatter factor; SF

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.

Product Specifications

Recombinant Human Hepatocyte growth factor (HGF) (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 32-728aa, with C-terminal 6xHis-tagged tag, molecular weight 79.7 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed:20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed:15167892, PubMed:20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed:20977675)

Gene: HGF  |  Organism: Homo sapiens

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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