Recombinant Human coronavirus NL63 Membrane protein (M) Protein, Human coronavirus NL63 (HCoV-NL63), in vitro E.coli expression system, N-terminal 10xHis-tagged, 28.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human coronavirus NL63 Membrane protein (M) Protein

Recombinant Human coronavirus NL63 Membrane protein (M) Protein — recombinant protein from Human coronavirus NL63 (HCoV-NL63), expressed in in vitro E.coli expression system. Purity >85%.

SKU: BCRECP-00038TM Categories: , , ,
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Product Specifications

Uniprot No.Q6Q1R9
Gene NamesM
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-226aa
SpeciesHuman coronavirus NL63 (HCoV-NL63)
Tag InfoN-terminal 10xHis-tagged
Molecular weight28.7kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins.

Biological Context

Subcellular Location: Virion membrane (Multi-pass membrane protein); Host Golgi apparatus membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Human coronavirus NL63 Membrane protein (M) Protein is a recombinant protein from Human coronavirus NL63 (HCoV-NL63), expressed in in vitro E.coli expression system, covering amino acids 1-226aa, with N-terminal 10xHis-tagged tag, molecular weight 28.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins

Subcellular Location

Virion membrane (Multi-pass membrane protein); Host Golgi apparatus membrane (Multi-pass membrane protein)

Subunit

Homomultimer. Interacts with envelope E protein in the budding compartment of the host cell, which is located between endoplasmic reticulum and the Golgi complex. Forms a complex with HE and S proteins. Interacts with nucleocapsid N protein. This interaction probably participates in RNA packaging into the virus

Gene: M  |  Organism: Human coronavirus NL63  |  Synonyms: E1 glycoprotein; Matrix glycoprotein; Membrane glycoprotein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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