Recombinant Human Steroidogenic factor 1 (NR5A1) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 59.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Steroidogenic factor 1 (NR5A1) Protein

Recombinant Human Steroidogenic factor 1 (NR5A1) Protein — Transcriptional activator. Purity >85%.

SKU: BCRECP-00042TM Category:
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Product Specifications

Uniprot No.Q13285
Gene NamesNR5A1
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-461aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight59.1kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: High expressed in the adrenal cortex, the ovary, the testis, and the spleen
Disease Association: 46,XY sex reversal 3 (SRXY3) : A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. [The disease is caused by variants affecting the gene represented in this entry] | 46,XX sex reversal 4 (SRXX4) : A condition in which male gonads develop in a genetic female (female to male sex reversal). [The disease is caused by variants affecting the gene represented in this entry] | Adrenal insufficiency, NR5A1-related (AINR) : A disorder characterized by adrenal insufficiency, muscular hypotonia, decreased sodium and increased potassium levels, elevated ACTH, salt-wasting crisis, prolonged jaundice, hypoglycemia, and vomiting. [The disease is caused by variants affecting the gene represented in this entry] | Premature ovarian failure 7 (POF7) : An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. [The disease is caused by variants affecting the gene represented in this entry] | Spermatogenic failure 8 (SPGF8) : An infertility disorder characterized by spermatogenesis failure and severe oligozoospermia. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Steroidogenic factor 1 (NR5A1) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 1-461aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 59.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed:27378692). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed:27378692). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation

Subcellular Location

Nucleus

Disease Association

46,XY sex reversal 3 (SRXY3) : A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. [The disease is caused by variants affecting the gene represented in this entry] | 46,XX sex reversal 4 (SRXX4) : A condition in which male gonads develop in a genetic female (female to male sex reversal). [The disease is caused by variants affecting the gene represented in this entry] | Adrenal insufficiency, NR5A1-related (AINR) : A disorder characterized by adrenal insufficiency, muscular hypotonia, decreased sodium and increased potassium levels, elevated ACTH, salt-wasting crisis, prolonged jaundice, hypoglycemia, and vomiting. [The disease is caused by variants affecting the gene represented in this entry] | Premature ovarian failure 7 (POF7) : An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. [The disease is caused by variants affecting the gene represented in this entry] | Spermatogenic failure 8 (SPGF8) : An infertility disorder characterized by spermatogenesis failure and severe oligozoospermia. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

High expressed in the adrenal cortex, the ovary, the testis, and the spleen (PubMed:9177385)

Subunit

Binds DNA as a monomer. Interacts with NR0B2 and PPARGC1A (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NCOA2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3

Gene: NR5A1  |  Organism: Homo sapiens  |  Synonyms: Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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