Recombinant Human Receptor activity-modifying protein 3 (RAMP3) Protein, Homo sapiens (Human), in vitro E.coli expression system, N-terminal 10xHis-tagged, 15.6kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Receptor activity-modifying protein 3 (RAMP3) Protein

Recombinant Human Receptor activity-modifying protein 3 (RAMP3) Protein — Accessory protein that interacts with and modulates the function of G protein-coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL), calcitonin receptor (CALCR) and G… Purity >95%.

SKU: BCRECP-00241TM Category:
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Product Specifications

Uniprot No.O60896
Gene NamesRAMP3
PurityGreater than 95% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region24-148aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight15.6kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Accessory protein that interacts with and modulates the function of G protein-coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL), calcitonin receptor (CALCR) and G protein-coupled estrogen receptor 1 (GPER1). Required for the transport of CALCRL and GPER1 receptors to the plasma membrane. Plays a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a GPER1-dependent manner.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Membrane (Single-pass type I membrane protein)
Tissue Specificity: Strongly expressed in lung, breast, immune system and fetal tissues

Product Specifications

Recombinant Human Receptor activity-modifying protein 3 (RAMP3) Protein is a recombinant protein from Homo sapiens (Human), expressed in in vitro E.coli expression system, covering amino acids 24-148aa, with N-terminal 10xHis-tagged tag, molecular weight 15.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Accessory protein that interacts with and modulates the function of G protein-coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL), calcitonin receptor (CALCR) and G protein-coupled estrogen receptor 1 (GPER1) (PubMed:23674134, PubMed:9620797). Required for the transport of CALCRL and GPER1 receptors to the plasma membrane (PubMed:23674134, PubMed:9620797). Plays a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a GPER1-dependent manner (PubMed:23674134). Together with CALCRL, form a receptor complex for adrenomedullin/ADM and intermedin/ADM2 (PubMed:32296767). Together with CALCR, act as a receptor complex for amylin/IAPP (PubMed:35324283)

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Membrane (Single-pass type I membrane protein)

Tissue Specificity

Strongly expressed in lung, breast, immune system and fetal tissues

Subunit

Heterodimer of CALCRL and RAMP3; interaction induces allosteric modulation of CALCRL function and ligand specificity for adrenomedullin/ADM and intermedin/ADM2 (PubMed:32296767). Heterodimer of CALCR and RAMP3; interaction form the receptor complex AMYR3 for amylin/IAPP (PubMed:35324283). Interacts with GPER1 (PubMed:23674134)

Gene: RAMP3  |  Organism: Homo sapiens  |  Synonyms: Calcitonin-receptor-like receptor activity-modifying protein 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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