Recombinant Mouse Tyrosinase (Tyr) Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 10xHis-tagged, 61.3kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Tyrosinase (Tyr) Protein

Recombinant Mouse Tyrosinase (Tyr) Protein — This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Purity >85%.

SKU: BCRECP-00280TM Category:
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Product Specifications

Uniprot No.P11344
Gene NamesTyr
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region19-533aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight61.3kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.

Biological Context

Subcellular Location: Melanosome membrane (Single-pass type I membrane protein); Melanosome
Tissue Specificity: Expressed in the skin
Disease Association: [Defects in Tyr result in various forms of albinism. Himalayan strain tyrosinase is temperature-sensitive]

Product Specifications

Recombinant Mouse Tyrosinase (Tyr) Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 19-533aa, with N-terminal 10xHis-tagged tag, molecular weight 61.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds (PubMed:2517217). Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (PubMed:1537333, PubMed:2517217, PubMed:35469906). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:1537333)

Subcellular Location

Melanosome membrane (Single-pass type I membrane protein); Melanosome

Disease Association

[Defects in Tyr result in various forms of albinism. Himalayan strain tyrosinase is temperature-sensitive]

Tissue Specificity

Expressed in the skin

Subunit

Forms an OPN3-dependent complex with DCT in response to blue light in melanocytes

Gene: Tyr  |  Organism: Mus musculus  |  Synonyms: Albino locus protein; Monophenol monooxygenase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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