Recombinant Mouse Melanocyte-stimulating hormone receptor (Mc1r)-VLPs Protein, Mus musculus (Mouse), Mammalian cell, C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions), 36.6kDa
,

Recombinant Mouse Melanocyte-stimulating hormone receptor (Mc1r)-VLPs Protein

Recombinant Mouse Melanocyte-stimulating hormone receptor (Mc1r)-VLPs Protein — G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and adrenocorticotropic hormone/ACTH, which are peptide products of the POMC precursor protein.

SKU: BCRECP-00408TM Categories: ,
Online Inquiry →

Product Specifications

Uniprot No.Q01727
Gene NamesMc1r
Expression SystemMammalian cell
Expression Region1-315aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions)
Molecular weight36.6kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and adrenocorticotropic hormone/ACTH, which are peptide products of the POMC precursor protein. Upon activation, MC1R couples with the G(s) protein, stimulating adenylate cyclase and activating the cAMP-dependent signaling pathway. This activation promotes melanogenesis, resulting in the production of eumelanin (black/brown) and pheomelanin (red/yellow) in melanocytes.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Mouse Melanocyte-stimulating hormone receptor (Mc1r)-VLPs Protein is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 1-315aa, with C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions) tag, molecular weight 36.6kDa. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and adrenocorticotropic hormone/ACTH, which are peptide products of the POMC precursor protein (PubMed:1325670). Upon activation, MC1R couples with the G(s) protein, stimulating adenylate cyclase and activating the cAMP-dependent signaling pathway. This activation promotes melanogenesis, resulting in the production of eumelanin (black/brown) and pheomelanin (red/yellow) in melanocytes. MC1R interacts with G protein-coupled receptor opsin 3/OPN3, which couples to G(i) proteins and inhibits the alpha-MSH-induced cAMP response, thereby reducing melanin synthesis (By similarity). Binding to Agouti/ASP precludes alpha-MSH-induced signaling, thereby downregulating melanogenesis (PubMed:7935841). Additionally, interaction with MGRN1 displaces the G(s) protein, further suppressing MC1R signaling (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Interacts with MGRN1, but does not undergo MGRN1-mediated ubiquitination; this interaction competes with GNAS-binding and thus inhibits agonist-induced cAMP production. Interacts with OPN3; the interaction results in a decrease in MC1R-mediated cAMP signaling and ultimately a decrease in melanin production in melanocytes. Interacts with GNB1, the interaction is important for coupling of MC1R to G protein

Gene: Mc1r  |  Organism: Mus musculus  |  Synonyms: Melanocortin receptor 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top