Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein, Bacillus subtilis (strain 168), in vitro E.coli expression system, N-terminal 6xHis-SUMO-tagged, 35.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein

Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein — Involved in biofilm formation. Purity >90%.

SKU: BCRECP-00604TM Category:
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Product Specifications

Uniprot No.P71014
Gene NamesbslA
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-181aa
SpeciesBacillus subtilis (strain 168)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight35.3kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Involved in biofilm formation. Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm. The layer is stable and capable of resistance to high mechanical force compression.

Biological Context

Subcellular Location: Secreted; Secreted, cell wall

Product Specifications

Recombinant Bacillus subtilis Biofilm-surface layer protein A (bslA) Protein is a recombinant protein from Bacillus subtilis (strain 168), expressed in in vitro E.coli expression system, covering amino acids 1-181aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 35.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882)

Subcellular Location

Secreted; Secreted, cell wall

Subunit

Forms polymers

Gene: bslA  |  Organism: Bacillus subtilis (strain 168)  |  Synonyms: ORF-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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