Recombinant Yersinia enterocolitica Protein YopB (yopB) Protein, Yersinia enterocolitica, in vitro E.coli expression system, N-terminal 6xHis-SUMO-tagged, 57.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Yersinia enterocolitica Protein YopB (yopB) Protein

Recombinant Yersinia enterocolitica Protein YopB (yopB) Protein — Essential for the establishment of Yersinia infections in a mouse model system, but not for the targeting of effector Yops. Purity >90%.

SKU: BCRECP-00611TM Category:
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Product Specifications

Uniprot No.P37131
Gene NamesyopB
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-401aa
SpeciesYersinia enterocolitica
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight57.9kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Essential for the establishment of Yersinia infections in a mouse model system, but not for the targeting of effector Yops. May modulate the host's immune response at a distance from the site of infection.

Biological Context

Subcellular Location: Secreted; Host membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Yersinia enterocolitica Protein YopB (yopB) Protein is a recombinant protein from Yersinia enterocolitica, expressed in in vitro E.coli expression system, covering amino acids 1-401aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 57.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Essential for the establishment of Yersinia infections in a mouse model system, but not for the targeting of effector Yops (PubMed:10209737). May modulate the host's immune response at a distance from the site of infection (PubMed:10209737)

Subcellular Location

Secreted; Host membrane (Multi-pass membrane protein)

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569). This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:10476031, PubMed:10581252, PubMed:22001511). Interacts with YopD/SctB (PubMed:22001511, PubMed:9627954). Together with YopD/SctB, forms a multimeric integral membrane complex with a mass of between 500 and 700 kDa (PubMed:22001511)

Gene: sctE  |  Organism: Yersinia enterocolitica  |  Synonyms: Translocator YopB; Yersinia outer protein B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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