Recombinan Rotavirus A Non-structural glycoprotein 4, partial Protein, Rotavirus A (isolate RVA/Cow/United States/B223/1983/G10P8[11 ]) (RV-A), in vitro E.coli expression system, N-terminal 6xHis-tagged and C-terminal Myc-tagged, 21.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinan Rotavirus A Non-structural glycoprotein 4, partial Protein

Recombinan Rotavirus A Non-structural glycoprotein 4, partial Protein — The secreted form acts as an enterotoxin that causes phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Purity >85%.

SKU: BCRECP-00713TM Categories: , ,
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Product Specifications

Uniprot No.Q9PYC8
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region52-175aa
SpeciesRotavirus A (isolate RVA/Cow/United States/B223/1983/G10P8[11 ]) (RV-A)
Tag InfoN-terminal 6xHis-tagged and C-terminal Myc-tagged
Molecular weight21.7kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

The secreted form acts as an enterotoxin that causes phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea.

Biological Context

Subcellular Location: Host rough endoplasmic reticulum membrane (Single-pass type III membrane protein); Host membrane, host caveola (Single-pass type III membrane protein); Secreted

Product Specifications

Recombinan Rotavirus A Non-structural glycoprotein 4, partial Protein is a recombinant protein from Rotavirus A (isolate RVA/Cow/United States/B223/1983/G10P8[11 ]) (RV-A), expressed in in vitro E.coli expression system, covering amino acids 52-175aa, with N-terminal 6xHis-tagged and C-terminal Myc-tagged tag, molecular weight 21.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

The secreted form acts as an enterotoxin that causes phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with host plasma membrane receptors on neighboring epithelial cells such as integrins ITGA1/ITGB1 and ITGA2/ITGB1

Subcellular Location

Host rough endoplasmic reticulum membrane (Single-pass type III membrane protein); Host membrane, host caveola (Single-pass type III membrane protein); Secreted

Subunit

Homotetramer. Interacts with the immature particle in the viroplasm. Interacts with host CAV1, early and late in infection. Interacts with host integrin ITGA1/ITGB1 heterodimer. Interacts with host integrin ITGA2/ITGB1 heterodimer. Interaction with microtubules blocks trafficking to the Golgi apparatus

Organism: Rotavirus A (isolate RVA/Cow/United States/B223/1983/G10P8[11])  |  Synonyms: NCVP5; NS28

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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