Recombinant Human Prostatic acid phosphatase (ACP3), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged, 46.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Prostatic acid phosphatase (ACP3), partial

Recombinant Human Prostatic acid phosphatase (ACP3), partial — (Microbial infection) Forms amyloid beta-sheet fibrils in semen. Purity >90%.

SKU: BCRECP-000019 Categories: ,
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Product Specifications

Product SkuBCRECP-000019
Product DescriptionRecombinant Human Prostatic acid phosphatase (ACP3) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 33-382aa. [Accession | P15309].
Uniprot No.P15309
Gene NamesACP3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region33-382aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight46.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

(Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Lysosome membrane (Single-pass type I membrane protein); Nucleus; Cytoplasm, cytosol
Tissue Specificity: Widely expressed. Expressed in the sarcolemma of skeletal muscle

Product Specifications

Recombinant Human Prostatic acid phosphatase (ACP3), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 33-382aa, with N-terminal 10xHis-tagged tag, molecular weight 46.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed:18083097, PubMed:19451623, PubMed:19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed:19451623). Assembly of SEVI amyloid fibrils and their amplification of HIV infection is inhibited by WW61, a non-natural hexapeptide inhibitor (Trp-His-Lys-chAla-Trp-hydroxyTic) (PubMed:21677644). The target cell attachment and enhancement of HIV infection is also inhibited by surfen (PubMed:19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed:19403677)

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Lysosome membrane (Single-pass type I membrane protein); Nucleus; Cytoplasm, cytosol

Tissue Specificity

Widely expressed. Expressed in the sarcolemma of skeletal muscle

Subunit

Homodimer; dimer formation is required for phosphatase activity

Gene: ACP3  |  Organism: Homo sapiens  |  Synonyms: 5'-nucleotidase; Acid phosphatase 3; Ecto-5'-nucleotidase; Protein tyrosine phosphatase ACP3; Thiamine monophosphatase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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