Recombinant Human Adhesion G protein-coupled receptor F5 (ADGRF5), partial, Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 39.7kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human Adhesion G protein-coupled receptor F5 (ADGRF5), partial

Recombinant Human Adhesion G protein-coupled receptor F5 (ADGRF5), partial — Adhesion G protein-coupled receptor. Purity >95%.

SKU: BCRECP-000039 Category:
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Product Specifications

Product SkuBCRECP-000039
Product DescriptionRecombinant Human Adhesion G protein-coupled receptor F5 (ADGRF5) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 227-565aa. [Accession | Q8IZF2].
Uniprot No.Q8IZF2
Gene NamesADGRF5
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region227-565aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight39.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Adhesion G protein-coupled receptor. In alveolar type II (ATII or AT2) cells, required for normal lung surfactant homeostasis. Modulation of both surfactant secretion and uptake by ATII cells is mediated by the downstream activation of GNAQ/GNA11 proteins and may be a consequence of increased cortical F-actin assembly induced by ADGRF5 activation.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in lung endothelial cells and in alveolar type II (ATII) cells (at protein level). Expressed high levels in subcutaneous adipose tissue in lean individuals and at lower levels in visceral fat. Expression levels in subcutaneous adipose tissue drastically drop in obese individuals

Product Specifications

Recombinant Human Adhesion G protein-coupled receptor F5 (ADGRF5), partial is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 227-565aa, with C-terminal 10xHis-tagged tag, molecular weight 39.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Adhesion G protein-coupled receptor (PubMed:28570277). In alveolar type II (ATII or AT2) cells, required for normal lung surfactant homeostasis (PubMed:28570277). Modulation of both surfactant secretion and uptake by ATII cells is mediated by the downstream activation of GNAQ/GNA11 proteins and may be a consequence of increased cortical F-actin assembly induced by ADGRF5 activation (PubMed:28570277). In the kidney, may play a role in the regulation of acid excretion into the primary urine, possibly by regulating the surface expression of V-ATPase proton pump (By similarity). As a receptor for soluble FNDC4 (sFNDC4), required for proper systemic glucose tolerance, specifically sensitizing white adipose tissue to insulin. Also plays a role in sFNDC4-induced decrease of local inflammation in white adipose tissue (PubMed:34016966)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in lung endothelial cells and in alveolar type II (ATII) cells (at protein level) (PubMed:23684610, PubMed:28570277). Expressed high levels in subcutaneous adipose tissue in lean individuals and at lower levels in visceral fat. Expression levels in subcutaneous adipose tissue drastically drop in obese individuals (PubMed:34016966)

Subunit

Homodimer; disulfide-linked. Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Fragment generates by the processing enzyme furin remains attached to the extracellular N-terminal fragment. Interacts (via N-terminal extracellular domain) with SFTPD

Gene: ADGRF5  |  Organism: Homo sapiens  |  Synonyms: G protein-coupled receptor 116
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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