Recombinant Human Chymotrypsin-like elastase family member 2A (CELA2A), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 29.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Chymotrypsin-like elastase family member 2A (CELA2A)

Recombinant Human Chymotrypsin-like elastase family member 2A (CELA2A) — Elastase that enhances insulin signaling and might have a physiologic role in cellular glucose metabolism. Purity >90%.

SKU: BCRECP-000347 Category:
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Product Specifications

Product SkuBCRECP-000347
Product DescriptionRecombinant Human Chymotrypsin-like elastase family member 2A (CELA2A) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 29-269aa. [Accession | P08217].
Uniprot No.P08217
Gene NamesCELA2A
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region29-269aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight29.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Elastase that enhances insulin signaling and might have a physiologic role in cellular glucose metabolism. Circulates in plasma and reduces platelet hyperactivation, triggers both insulin secretion and degradation, and increases insulin sensitivity.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in pancreas. Not detected in keratinocytes. Detected in exocrine secretions of the pancreas (at protein level). Also expressed in a small fraction of cells in pancreatic islets, adrenal cortex, intestinal glands and colonic lymphoid follicles (at protein level). Detected in plasma
Disease Association: Abdominal obesity-metabolic syndrome 4 (AOMS4) : A form of abdominal obesity-metabolic syndrome, a disorder characterized by abdominal obesity, high triglycerides, low levels of high density lipoprotein cholesterol, high blood pressure, and elevated fasting glucose levels. AOMS4 is an autosomal dominant disease. Patients manifest obesity, hypertension, early-onset coronary artery disease and type 2 diabetes. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Chymotrypsin-like elastase family member 2A (CELA2A) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 29-269aa, with N-terminal 6xHis-tagged tag, molecular weight 29.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Elastase that enhances insulin signaling and might have a physiologic role in cellular glucose metabolism. Circulates in plasma and reduces platelet hyperactivation, triggers both insulin secretion and degradation, and increases insulin sensitivity

Subcellular Location

Secreted

Disease Association

Abdominal obesity-metabolic syndrome 4 (AOMS4) : A form of abdominal obesity-metabolic syndrome, a disorder characterized by abdominal obesity, high triglycerides, low levels of high density lipoprotein cholesterol, high blood pressure, and elevated fasting glucose levels. AOMS4 is an autosomal dominant disease. Patients manifest obesity, hypertension, early-onset coronary artery disease and type 2 diabetes. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in pancreas. Not detected in keratinocytes (PubMed:10620133). Detected in exocrine secretions of the pancreas (at protein level). Also expressed in a small fraction of cells in pancreatic islets, adrenal cortex, intestinal glands and colonic lymphoid follicles (at protein level) (PubMed:31358993). Detected in plasma (PubMed:31358993)

Subunit

Interacts with CPA1. Interacts with SERPINA1 (PubMed:31358993)

Gene: CELA2A  |  Organism: Homo sapiens  |  Synonyms: Elastase-2A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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