Recombinant Human Diacylglycerol kinase alpha (DGKA), Homo sapiens (Human), E.coli, N-terminal 6XHis-tagged, 86.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Diacylglycerol kinase alpha (DGKA)

Recombinant Human Diacylglycerol kinase alpha (DGKA) — Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Purity >90%.

SKU: BCRECP-000502 Categories: , ,
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Product Specifications

Product SkuBCRECP-000502
Product DescriptionRecombinant Human Diacylglycerol kinase alpha (DGKA) Protein is expressed from E.coli with N-terminal 6XHis-tagged. It contains 1-735aa. [Accession | P23743].
Uniprot No.P23743
Gene NamesDGKA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-735aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6XHis-tagged
Molecular weight86.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable).

Biological Context

Subcellular Location: Cytoplasm, cytosol
Tissue Specificity: Expressed in lymphocytes
Pathway: Lipid metabolism; glycerolipid metabolism

Product Specifications

Recombinant Human Diacylglycerol kinase alpha (DGKA) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-735aa, with N-terminal 6XHis-tagged tag, molecular weight 86.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:2175712). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:2175712). Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348). Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129)

Subcellular Location

Cytoplasm, cytosol

Tissue Specificity

Expressed in lymphocytes

Subunit

Monomer

Pathway

Lipid metabolism; glycerolipid metabolism

Gene: DGKA  |  Organism: Homo sapiens  |  Synonyms: 80 kDa diacylglycerol kinase; Diglyceride kinase alpha
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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