Recombinant Human Extracellular matrix protein 1 (ECM1), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged, 52.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Extracellular matrix protein 1 (ECM1), partial

Recombinant Human Extracellular matrix protein 1 (ECM1), partial — Involved in endochondral bone formation as negative regulator of bone mineralization. Purity >85%.

SKU: BCRECP-000554 Category:
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Product Specifications

Product SkuBCRECP-000554
Product DescriptionRecombinant Human Extracellular matrix protein 1 (ECM1) Protein is expressed from E.coli with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged. It contains 386-540aa. [Accession | Q16610].
Uniprot No.Q16610
Gene NamesECM1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region386-540aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Molecular weight52.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix
Tissue Specificity: Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis
Disease Association: Lipoid proteinosis (LiP) : Rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Extracellular matrix protein 1 (ECM1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 386-540aa, with N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged tag, molecular weight 52.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity

Subcellular Location

Secreted, extracellular space, extracellular matrix

Disease Association

Lipoid proteinosis (LiP) : Rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis

Subunit

Interacts (via C-terminus) with HSPG2 (via C-terminus). Interacts with EFEMP1/FBLN3 and LAMB3. Interacts with MMP9

Gene: ECM1  |  Organism: Homo sapiens  |  Synonyms: Secretory component p85
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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