Recombinant Human F-box only protein 44 (FBXO44), Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 37.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human F-box only protein 44 (FBXO44)

Recombinant Human F-box only protein 44 (FBXO44) — Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex. Purity >85%.

SKU: BCRECP-000639 Category:
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Product Specifications

Product SkuBCRECP-000639
Product DescriptionRecombinant Human F-box only protein 44 (FBXO44) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-255aa. [Accession | Q9H4M3].
Uniprot No.Q9H4M3
Gene NamesFBXO44
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-255aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight37.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areascell Biology

Function

Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex. The complex SCF(FBXO44) complex ubiquitinates and mediates BRCA1 degradation. In association with cullin 4B/CUL4B and DDB1, mediates the ubiquitination of RGS2 leading to its degradation.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver

Product Specifications

Recombinant Human F-box only protein 44 (FBXO44) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-255aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 37.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex (PubMed:18203720, PubMed:25970626). The complex SCF(FBXO44) complex ubiquitinates and mediates BRCA1 degradation (PubMed:23086937). In association with cullin 4B/CUL4B and DDB1, mediates the ubiquitination of RGS2 leading to its degradation (PubMed:25970626). Additionally, regulates the pregnane X receptor/NR1I2 protein level by ubiquitination and degradation (PubMed:37969738)

Subcellular Location

Nucleus

Tissue Specificity

Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver

Subunit

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1 and CUL1. Interacts with CUL4B and DDB1 (PubMed:25970626). Interacts wwith BRCA1; this interaction mediates BRCA1 ubiquitination and subsequent degradation (PubMed:23086937). Interacts with RGS2; this interaction mediates RGS2 ubiquitination and subsequent degradation (PubMed:25970626). Interacts with PXR/NR1I2; this interaction mediates PXR/NR1I2 ubiquitination and subsequent degradation (PubMed:37969738)

Gene: FBXO44  |  Organism: Homo sapiens  |  Synonyms: F-box protein FBX30; F-box/G-domain protein 3
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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