Recombinant Human Zinc finger protein GLI1 (GLI1), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 23.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Zinc finger protein GLI1 (GLI1), partial

Recombinant Human Zinc finger protein GLI1 (GLI1), partial — Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Purity >90%.

SKU: BCRECP-000754 Category:
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Product Specifications

Product SkuBCRECP-000754
Product DescriptionRecombinant Human Zinc finger protein GLI1 (GLI1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 921-1106aa. [Accession | P08151].
Uniprot No.P08151
Gene NamesGLI1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region921-1106aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight23.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Undetectable in normal cells but highly expressed in cancer cells
Disease Association: Polydactyly, postaxial, A8 (PAPA8) : A form of postaxial polydactyly, a condition characterized by the occurrence of supernumerary digits in the upper and/or lower extremities. In postaxial polydactyly type A, the extra digit is well-formed and articulates with the fifth or a sixth metacarpal/metatarsal. PAPA8 is an autosomal recessive condition characterized by the presence of postaxial extra digits (hexadactyly) on the hands and/or the feet. [The disease is caused by variants affecting the gene represented in this entry] | Polydactyly, preaxial 1 (PPD1) : A form of polydactyly, a condition defined by the occurrence of supernumerary digits in the upper and/or lower extremities. Preaxial or radial polydactyly refers to the presence of extra digits on the radial side of the hand. PPD1 is an autosomal recessive form characterized by duplication of the distal phalanx of the thumb. [The disease may be caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Zinc finger protein GLI1 (GLI1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 921-1106aa, with N-terminal 6xHis-tagged tag, molecular weight 23.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as a transcriptional activator, but activates a different set of genes than isoform 1 (PubMed:19706761). Activates expression of CD24, unlike isoform 1 (PubMed:19706761)

Subcellular Location

Cytoplasm; Nucleus

Disease Association

Polydactyly, postaxial, A8 (PAPA8) : A form of postaxial polydactyly, a condition characterized by the occurrence of supernumerary digits in the upper and/or lower extremities. In postaxial polydactyly type A, the extra digit is well-formed and articulates with the fifth or a sixth metacarpal/metatarsal. PAPA8 is an autosomal recessive condition characterized by the presence of postaxial extra digits (hexadactyly) on the hands and/or the feet. [The disease is caused by variants affecting the gene represented in this entry] | Polydactyly, preaxial 1 (PPD1) : A form of polydactyly, a condition defined by the occurrence of supernumerary digits in the upper and/or lower extremities. Preaxial or radial polydactyly refers to the presence of extra digits on the radial side of the hand. PPD1 is an autosomal recessive form characterized by duplication of the distal phalanx of the thumb. [The disease may be caused by variants affecting the gene represented in this entry]

Tissue Specificity

Undetectable in normal cells but highly expressed in cancer cells

Subunit

Interacts with SUFU; sequestering GLI1 in the cytoplasm (PubMed:10806483, PubMed:10559945, PubMed:24217340, PubMed:24311597, PubMed:28965847). Interacts with KIF7 (By similarity). Interacts with STK36 (PubMed:10806483). Interacts with ZIC1; the interaction enhances transcription activation (PubMed:11238441)

Gene: GLI1  |  Organism: Homo sapiens  |  Synonyms: Glioma-associated oncogene; Oncogene GLI
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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