Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 70.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A)

Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A) — Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Purity >85%.

SKU: BCRECP-001133 Categories: ,
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Product Specifications

Product SkuBCRECP-001133
Product DescriptionRecombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-395aa. [Accession | P31153].
Uniprot No.P31153
Gene NamesMAT2A
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-395aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight70.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Biological Context

Tissue Specificity: Detected in kidney
Pathway: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1

Product Specifications

Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-395aa, with N-terminal GST-tagged tag, molecular weight 70.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate

Tissue Specificity

Detected in kidney

Subunit

Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain (PubMed:10644686, PubMed:23189196). Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain (PubMed:25075345)

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1

Gene: MAT2A  |  Organism: Homo sapiens  |  Synonyms: Methionine adenosyltransferase 2; Methionine adenosyltransferase II
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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