Recombinant Human D-3-phosphoglycerate dehydrogenase (PHGDH), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-GST-tagged, 58.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human D-3-phosphoglycerate dehydrogenase (PHGDH), partial

Recombinant Human D-3-phosphoglycerate dehydrogenase (PHGDH), partial — Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Purity >85%.

SKU: BCRECP-001379 Categories: ,
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Product Specifications

Product SkuBCRECP-001379
Product DescriptionRecombinant Human D-3-phosphoglycerate dehydrogenase (PHGDH) Protein is expressed from E.coli with N-terminal 6xHis-GST-tagged. It contains 2-251aa. [Accession | O43175].
Uniprot No.O43175
Gene NamesPHGDH
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-251aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-GST-tagged
Molecular weight58.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

Biological Context

Disease Association: Phosphoglycerate dehydrogenase deficiency (PHGDHD) : An autosomal recessive inborn error of L-serine biosynthesis, clinically characterized by congenital microcephaly, psychomotor retardation, and seizures. [The disease is caused by variants affecting the gene represented in this entry] | Neu-Laxova syndrome 1 (NLS1) : A lethal, autosomal recessive multiple malformation syndrome characterized by ichthyosis, marked intrauterine growth restriction, microcephaly, short neck, limb deformities, hypoplastic lungs, edema, and central nervous system anomalies including lissencephaly, cerebellar hypoplasia and/or abnormal/agenesis of the corpus callosum. Abnormal facial features include severe proptosis with ectropion, hypertelorism, micrognathia, flattened nose, and malformed ears. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3

Product Specifications

Recombinant Human D-3-phosphoglycerate dehydrogenase (PHGDH), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-251aa, with N-terminal 6xHis-GST-tagged tag, molecular weight 58.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate

Disease Association

Phosphoglycerate dehydrogenase deficiency (PHGDHD) : An autosomal recessive inborn error of L-serine biosynthesis, clinically characterized by congenital microcephaly, psychomotor retardation, and seizures. [The disease is caused by variants affecting the gene represented in this entry] | Neu-Laxova syndrome 1 (NLS1) : A lethal, autosomal recessive multiple malformation syndrome characterized by ichthyosis, marked intrauterine growth restriction, microcephaly, short neck, limb deformities, hypoplastic lungs, edema, and central nervous system anomalies including lissencephaly, cerebellar hypoplasia and/or abnormal/agenesis of the corpus callosum. Abnormal facial features include severe proptosis with ectropion, hypertelorism, micrognathia, flattened nose, and malformed ears. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Homotetramer

Pathway

Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3

Gene: PHGDH  |  Organism: Homo sapiens  |  Synonyms: 2-oxoglutarate reductase; Malate dehydrogenase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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